In vitro transformation assay of major fractions of cigarette smoke condensate (CSC) in mammalian cell lines.

Abstract

Discussion and SummaryIt is interesting that the data from our in vitro studies corroborate the in vivo results obtained by Bock, Swain and Stedman (16). Bock, Swain and Stedman (16) reported that the weak acid (phenol) fraction (No. 8) and two neutral fractions (Nos. 13 and 14) had statistically significant tumor inducing activity. In addition, the third neutral fraction, 12, gave results suggesting activity, and the ether-soluble base fraction (No. 5) exhibited possible activity. We found cell transforming activity with four fractions (Nos. 3, 8, 13 and 14) of CSC. Fractions 8 and 14 exhibited early transformation in infected mouse and rat cells and also subsequently in uninfected hamster cells suggesting that they were most active. Fraction 13 showed transformation in infected mouse cells and uninfected hamster cells. In addition, fraction 3 demonstrated changes in infected mouse and rat cells. However, none of the fractions exhibited cell alteration in uninfected mouse and rat cells. These findings ar...