Abstract
This experimental research determined the feasibility of micropropagating in vitro,broccoli from its curd through plant tissue culture technology to be able to produceplants that are free from any disease. The growth of broccoli (Brassica oleraceavar Italica curd in vitro was measured in terms of organ formation and the cultures’average height (cm) after the observation period. It also determined the percentsurvivorship of the plant cultures after the observation period. The method usedwas adopted from Balcita (2011) utilizing the modified Murashige and Skoog (MS)medium. Result of the study show that broccoli plantlets grew from the curd after asix-week observation period. Addition of thiamine in the culture medium protectedthe cultures from bacterial or fungal infections, thus coming up with very healthyplantlets and a high survivorship of the cultures was also observed. In conclusion,propagation of broccoli curd in vitro is feasible using the modified Murashige andSkoog (MS) medium; plantlets with roots, stem, leaves, and curd with florets can grow directly from the broccoli curd; and there is a high percent survivorship in thecultures with the addition of thiamine in the culture medium.
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