Abstract
Tetraploid plants were produced from leaf explants of diploid Populus pseudo-simonii by treating the leaves with colchicine. Leaf explants were cultured on MS basal medium containing 1.78μM BA and 1.08μM NAA for 0, 6 and 12days, and then transferred to the same MS liquid medium with colchicine at concentrations of 25, 50 and 75μM for 1, 2 and 3days. The highest efficiency of tetraploid induction was 14.6% by treating leaf explants that were pre-cultured for 6days and then cultured in liquid MS with 50μM colchicine for 3days. Flow cytometric analysis was used to screen the tetraploids out from the regenerated plants and chromosome number counting was employed to confirm the polyploidy level. Size and frequency of leaf stomata between diploid and tetraploid plants were demonstrated to have significant differences.
Published Version
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