Abstract
Two new simple in vitro screening tests are described which have proved valuable in predicting the degerming effectiveness of germicidal toilet bars under regular use conditions. The tests, skin disk substantivity and finger imprint tests, measure the residual antibacterial activity of skin treated with soap solutions followed by a water rinse. Thus, a soap germicide is evaluated on the basis of its capacity to become adsorbed to skin and its ability, at low concentrations, to inhibit the growth of bacteria. In vitro test data are described on 3,4',5-tribromosalicylanilide and other germicides incorporated into soap and detergent bars. Good correlation between the new in vitro test results and regular hand washing results (serial basin wash test) is achieved.
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