In vitro targeting and selective killing of mcf-7 and colo320dm cells by 5-fluorouracil anchored to carboxylated SWCNTs and MWCNTs

Rutuja V Kamble,Somnath D Bhinge,Shrinivas K Mohite,Dheeraj S Randive,Mangesh A Bhutkar

doi:10.1007/s10856-021-06540-8

Abstract

The intention of the present work was to synthesize the f-MWCNT and f-SWCNT terminated with proper functional group, loading of 5-Flurouracil and to perform cytotoxic activity. Functionalization of MWCNTs and SWCNTs was achieved through the acid treatment (H2SO4 + HNO3). 5-flurouracil was loaded into the prepared functionalized CNTs, thereafter; in vitro drug loading capacity and % drug release were calculated. Also the prepared f-CNTs, 5-flurouracil loaded CNTs were distinguished by using SEM, TGA, DSC, X-ray diffraction, Raman and FTIR spectroscopy. MCF-7 and COLO320DM cells were treated with selected concentrations of 5-FU loaded f-MWCNTs and f-SWCNTs to estimate the cytotoxic activity. It was observed that 5-FU loaded f-SWCNTs showed good activity against selected cell lines than others. Moreover, apoptosis percentage was reported to be 84.46 ± 4.3515 and 92.78 ± 2.6549 for 5-FU loaded f-SWCNTs against MCF-7 and COLO320DM cells respectively. It is evident from the results that the prepared drug loaded CNTs have comparable antitumor activity in cancer cell lines.

Highlights

The results clearly indicated that 5-FU loaded MWCNTs and 5-FU loaded SWCNTs induced apoptosis in MCF-7 and COLO320DM cells when compared with control (Fig. 10)
Probable mechanism was verified with apoptosis studies, our study revealed that all studied parameters exhibited almost same results for both carbon nanotubes (CNTs), in apoptosis study 5-FU loaded f-SWCNTs showed the maximum apoptotic
MWCNTs and SWCNTs have been considered as lucrative options for the targeted and controlled drug delivery owing to their intrinsic properties

Summary

Introduction

The toxicities of combination drug therapy is high to the patients owing to an increase in the drug numbers in the formulation [4]. The cost of the anticancer agent(s) is very much high and an increase of drug number, will lead to an increase in the cost of the formulation. Paclitaxel and 5FU can be quoted as a classic example of fixed drug combination used in chemotherapy imparting good anticancer activity but they are interfering with each other [3, 5]. González-Lavado et al [3] have reported that 5-FU loaded in nanomaterial (MWCNTs) exhibited good therapeutic effect against the human and murine cancer cells [3]

Methods

Discussion

Conclusion