In vitro susceptibility and the resistance plasmids genptype of Neisseria gonorrhoeae isolates from Guangzhou and Dafian

Abstract

Objective To detect the antibiotic sensitivity of Neisseria gonorrhoeae isolated from Guangzhou and Dalian in China, and to analyze the TEM-1 and tetM genotypes of plasmid-mediated resistant strains of peni- cillinase （13-1actamase）-producing Neisseria gonorrhoeae （PPNG） and tetracycline-resistant Neisseria gonorrhoeae （TRNG）. Methods Neisseria gonorrhoeae strains were isolated from STD clinic patients of Guangdong Provincial Centers for Skin Diseases and STDs Control and Prevention and Dalian Dermatology Hospital. The agar dilution method was used to determine the drug resistance of strains to ciprofloxacin, ceftriaxone and spectinomycin. The multi-PCR was used to distinguish subtypes of TEM-1 and tetM. Results One hundred and twenty seven isolates of Neisseria gonorrhoeae from Guangzhou and 102 isolates from Dalian were collected in 2012. The ceftriaxone intermediate rate of Dalian isolates was 88.2%（90/102）, which was significantly higher than Guangzhou isolates of 46.5%（59/127,x^2=43.44, P〈0.01 ）. Five isolates （4.9%）from Dalian resisted to spectinomycin. The prevalence of PPNG and TRNG from Guangzhou were 46.5% （59/127）and 50.4% （64/127）, which were significantly higher than Dalian PPNG and TRNG of 33.3% （34/102）and 24.5% （25/102）, respectively （x^2=5.24, 15.95, P all〈0.05）. The TEM-1 genotype of the 2 cities mainly showed Asian-type plasmids with 84.7% （50/59） and 79.4% （27/34）, respectively. The tetM genotype dominated by Dutch variant, with 100.0% （64/64） and 92.0% （23/25） of Guangzhou and Dalian, respectively. Conclusions The Ne isseria gonorrhoeae strains isolated from Guangzhou and Dalian show different resistant rates. Spectinomycin resistant strains emerge in Dalian. Ceftriaxone intermediate rate of Dalian isolates is significantly higher than that of Guangzhou. Guangzhou PPNG and TRNG prevalence rates are significantly higher than Dalian. The TEM-1 and terM genotype are dominated by Asian-type and Dutch-type, respectively. Key words: Neisseria gonorrhoeae; Microbial sensitivity tests; Genotyping