Abstract
The aim of this study was to assess the dose- and time dependent in vitro effects of resveratrol (RES), a natural polyphenol and phytoalexin with potential antiviral, anti-inflammatory and antioxidant properties on bovine spermatozoa during five different time periods (0h, 2h, 6h, 12h and 24h). Semen samples were collected from 20 adult breeding bulls, and diluted in physiological saline solution containing 0.5% DMSO together with 0, 1, 5, 10, 50, 100 and 200 μM/L RES. Spermatozoa motility was examined using the Sperm VisionTM and CASA (Computer Assisted Semen Analyzer) system. Cell viability was measured using the metabolic activity MTT assay, the nitroblue-tetrazolium (NBT) test was used to assess the intracellular superoxide formation. The initial CASA analysis showed no significant changes in the spermatozoa motion parameters, however a motion-promoting effect of 10 and 50 μM/L RES became significant after 2h (P
Highlights
Uncontrolled overproduction of reactive oxygen species (ROS) followed by oxidative stress (OS) development has become a threat to male fertility (Agarwal et al, 2003)
The CASA assessment showed a continuous decrease of spermatozoa motility and progressive motility in all groups over the course of a 24h in vitro culture (Table 1, Table 2)
Examination at 12h of in vitro culture showed that the spermatozoa motility and progressive motility were significantly increased in groups C (P
Summary
Uncontrolled overproduction of reactive oxygen species (ROS) followed by oxidative stress (OS) development has become a threat to male fertility (Agarwal et al, 2003). The specific cellular structure of spermatozoa renders them to be vulnerable to oxidative damage. Their plasma membranes contain large quantities of polyunsaturated fatty acids (PUFA), while their cytoplasm contains low concentrations of scavenging enzymes (Tvrdá et al, 2011), leading to increased ROS attacks with a subsequent decrease in motility and viability (de Lamirande and Gagnon, 1992). Studies have shown that antioxidant supplementation may protect spermatozoa from ROS sources, directly scavenge ROS, prevent DNA fragmentation, improve semen quality, reduce cryodamage to spermatozoa, block premature sperm maturation and generally stimulate spermatozoa metabolism (Agarwal et al, 2007). This study was designed to evaluate the in vitro effects of resveratrol on ejaculated bovine spermatozoa in order to assess possible beneficial or toxic effects of this compound, as well as to define its efficacy for further experiments
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