In Vitro Study the Effects of Anti-Fungal Agents on the Mycelial Growth of Aspergillus Niger

Abstract

The purpose of this study was to determine the sensitivity of Aspergillus niger to antifungal agents (Terbinafine, Nystatin and Clotrimazole) by using several parameter include minimum inhibitory concentration (MIC), inhibition zone diameter and spores growth test. MIC value of Terbinafine showed highly effect compere with Clotrimazole and Nystatin. The in vitro study revealed that the Terbinafine have the potential to inhibit mycelial growth (inhibition zone diameter) of Aspergillus niger at concentrations of 1%, whereas Clotrimazole and Nystatin have lowest effect to inhibit mycelial growth of Aspergillus niger at concentrations of 1% and 100000 IU/ml respectively. Spore growth test for Terbinafine showed no growth of Aspergillus niger spores on SDA media through incubation in SDA at 28oC for 5 days, While Clotrimazole showed no growth of Aspergillus niger spores on SDA media in the 3ed days of incubation, and Nystatin showed no growth of Aspergillus niger spores in the 5th day of incubation at same condition.