In-Vitro Study on Permeation of different Semi-solid dosage forms of Timolol Maleate using Franz cell

Abstract

The aim of the present study was to formulate different semi-solids dosage form for Timolol Maleate (0.5%) in oleaginous ointments, hydrocarbon gel and hydrogels and to study in-vitro comparison of flux and permeability into cornea of these semi-solids dosage form in comparison to ophthalmic solution using Franz diffusion cell. Objective of the study compare rate and extent of steady state flux in between semi-solids and solution of timolol maleate and obtain permeation co-efficient of all formulations from steady state flux using Fick’s first law of diffusion. An modified Franz diffusion cell consisting of 20 ml glass receptor along with a glass donor was for used for study and analysis of permeation was carried out using high performance liquid chromatography at time points 0,0.5,1,2,4,8,16 and 24 h. Results concluded that hydrogel formulation containing hydroxy propyl methyl cellulose as an gelling agent was found to have better flux and permeability than the reference solution formulation, while other two formulation containing paraffin base had less flux and permeability than reference solution formulation.