In Vitro Study of Variables Relevant to Perioperative Care of the Surgical Patient: Glucose, Osmolarity, and Rewarming

Abstract

We sought to determine the effects of altering osmolarity and the reversibility of the detrimental immunologic effects of hypothermia on human monocyte HLA-DR surface expression and reactive oxygen species (ROS) formation. The effects of altering osmolarity on HLA-DR surface expression and ROS formation were assessed using lipopolysaccharide (LPS)-treated samples treated with either saline, glucose, or mannitol, incubated at 37°C for 2 hours. HLA-DR surface receptor expression and ROS formation were determined after incubation. The effects of the reversibility of hypothermia were measured by incubating LPS-treated samples at 34°C, 37°C, and 40°C for 1 hour. The samples were subsequently rewarmed at 40°C for 1 and 2 hours. The effects of rewarming on HLA-DR surface receptor expression and ROS formation were reassessed. In the osmolarity experiments, there was a 49% decrease in ROS formation in samples treated with mannitol as compared with saline and glucose. Alterations of osmolarity had no significant effect on HLA-DR surface expression. In the rewarming experiments, rewarming for either 1 or 2 hours abolished any significant differences in HLA-DR surface expression and ROS formation between samples preincubated at the different temperatures. The presumed inert mannitol was found to significantly decrease ROS formation, but had no effect on HLA-DR surface expression. In addition, the effects of hypothermia on HLA-DR surface expression or ROS formation may be better reversed within 2 hours than in 1 hour.