In-vitro study of the radioprotective effect of palladium α-lipoic acid nano-complex on hemoglobin molecule

Abstract

This work is intended to study the radioprotective effect of palladium α-lipoic acid nano-complex (PLAC) on hemoglobin molecule in vitro. Blood samples were obtained from adult male rats weighing 120–150 g after dissection, using heparinized needles. Each blood sample was divided into four groups; the first group was kept untreated as control, palladium α-lipoic acid (PLAC) was added to the second group at concentration 2% v/v, the third group was exposed to 100 Gy gamma radiation and the forth group was irradiated with the addition of PLAC. Hemoglobin was extracted and prepared for measurement. The effects on the hemoglobin molecule were evaluated by FTIR and UV–visible spectroscopy. The results showed that PLAC increases the optical energy gap of the transition of the amino acid side chains and affects the spatial distribution of the globin part. Gamma radiation affects mainly the globin part, results in unfolding of the protein structure and perturbation in the relative orientation of the transition dipole moments. Addition of PLAC to the blood samples prior to irradiation was shown to provide protective effects which can be attributed to its ability to neutralize the free radicals.