In vitro study of the effects of RNA binding motif protein 38 expression on the biological behavior of human breast cancer BT474 cells

Abstract

Objective To investigate the effect of RNA binding motif protein 38 (RNPC1) over-expression on the biological behavior of a human breast cancer cell line BT474.Methods Cell proliferation was tested by cell counting kit 8 (CCK-8) assay.The ability of colony formation was tested by plate clone formation assay.The abilities of migration and invasion were evaluated by Transwell chambers.The relative expression of matrix metalloproteinase-2 (MMP-2) protein was detected by Western blotting.Results CCK-8 test showed that the absorbance (A) value in experimental group,blank group and negative group at6th day was (0.714 7 ±0.018 9),(0.770 8 ±0.016 0) and (0.556 8 ±0.043 6),respective-ly (P ＜ 0.05).The colony formation number at 14th day was significantly less in experimental group (113.300 ±3.528) than in blank group (118.700 ±2.404) and negative group (47.330 ±2.906) (P ＜0.01).The Transwell assay revealed that the number of cells passing through the Transwell membrance in experimental group [(61.670 ±4.842) for migration,and (14.670 ± 1.856) for invation] was significantly less than in blank group and control group (both P ＜ 0.01).Overexpression of RNPC1 a down-regu-lated the expression of MMP-2 protein.Conclusion RNPC1a exhibits multiple anticancer functions in breast cancer cells.RNPC1a interacts with MMP-2 to play an important role in the metastasis of breast cancer. Key words: Breast cancer; RNA binding motif protein 38; Proliferation; Invasion