In- vitro Study of the Effect of Clotrimazole Incorporation into Silicone Soft Liner on Fungal Colonization

Abstract

Statement of Problem: Candidal colonization on soft liners is important in the development of pathogenesis, such as denture stomatitis. It has heen reported that combining anti-fungal agents into soft liners might be used in treatment and prevention of denture stomatitis. Purpose: The aim of this in-vitro study was to determine whether incorporating clotrimazole (C) into the silicone soft liner (S), would inhibit the growth of C. albicans when the specimens are stored in distilled water and washed daily with wet cotton. Methods and Materials: Experimental specimen disks incorporated with clotrimazole (Sc 1 , Sc 30 , Sc 60 ) and without clotrimazole (S 1 , S 30 , S 60 ) into the soft liner (no=8) were fabricated aseptically against the polyester film to produce a smooth surface. The treated and control disks were stored in distilled water for 1, 30 and 60 days and washed daily with wet cotton. For fungal growth assessment, they were inoculated with C. albicans suspension. The disks were rinsed and sonicated in sterile water to remove surface organisms. The attached yeast was measured by inoculation of the yeast suspension on Sabouraud's agar. The data were compared using two-way ANOVA. Results: The mean colony forming units (CFU) per mm 2 for the specimens without clotrimazole after water storage and washing with wet cotton for 1, 30 and 60 days was 6.5I10 6 , 5.8I10 6 and 6.1I10 6 , respectively. CFU for specimens with clotrimazole decreased significantly to 2.6I10 6 and 3.9I10 6 and 4.6I10 6 after 1, 30, and 60 days, respectively. In comparison to those of the control disks, clotrimazole in treated disks was effective in inhibiting C. albicans growth significantly following storage in water for 2 months ( P <0.05). Conclusion: The addition of clotrimazole significantly reduced C. albicans growth to the surface of the silicone soft liner. The samples continued to inhibit the fungal growth when they were washed daily with wet cotton for 2 months.