Abstract
Objective: This study aimed to compare the bronchorelaxation effect of the methanol extract, hexane fraction, ethyl acetate fraction, and methanol fraction of Coptosapelta flavescens Korth. roots (CFRs) on the isolated bronchial rings of guinea pig.
 Methods: Research design was experimental in which a 4-mm long bronchus of a male guinea pig was kept in an isolated organ bath containing Krebs-Henseleit solution at 37°C, pH 7.4, and flooded with carbogen gas. The bronchial ring was precontracted with methacholine and then given six doses of C. flavescens Korth. root’s methanol extract (MECFR) solution cumulatively. The extract’s solvent 10% dimethyl sulfoxide ethanol was used as negative control. This procedure was then repeated using CFRs hexane fraction (HFCFR) solution, CFRs ethyl acetate fraction (EAFCFR), and CFRs methanol fraction (MFCFR).
 Results: The bronchorelaxation effect of MECFR, MFCFR, EAFCFR, and HFCFR is shown by their dose-response curves (DRCs) which are significantly different compared with the extracts solvent’s DRC. The maximal efficacy (Emax) of MFCFR was the same (p>0.05) as the MECFRs Emax, but the EAFCFRs and HFCFRs Emax were smaller (p<0.05) than the MECFRs Emax.
 Conclusion: The bronchorelaxation effect of the MECFR on the guinea pig’s bronchial ring is similar to the CFRs methanol fraction, and is stronger than the CFRs ethyl acetate fraction and CFRs hexane fraction.
Highlights
Coptosapelta flavescens Korth. is a liana plant from Rubiaceae family
Yield from methanol extract, hexane fraction, ethyl acetate fraction and methanol fraction of Coptosapelta flavescens Korth. roots (CFRs) Table 1 shows the MECFR yield from 300 g of CFR simplicia and HFCFR, EAFCFR, and MFCFR yield from 667 g of CFR simplicia
The largest yield was obtained from MECFR (21.94%), while the least yield was from hexane fraction (HFCFR) and ethyl acetate fraction (EAFCFR) at only 4%
Summary
In Kalimantan, it is known as Akar Tambolekar or Akar Merung [1] or Akar Manuran [2] It is used in ethnobotany as herbs to overcome cough and shortness of breath [3]. It has been known that MECFR contains secondary metabolites of saponins, polyphenols, triterpenoids, steroids, and anthraquinone; CFRs hexane fraction (HFCFR) contains anthraquinone and steroids; CFRs ethyl acetate fraction (EAFCFR) contains polyphenols, terpenoids, and anthraquinone; and CFRs methanol fraction (MFCFR) contains saponins, polyphenols, and terpenoids [6]. While there were existing studies on MECFR bronchorelaxation effect on guinea pig’s bronchial ring precontracted with methacholine, there are no data available on the bronchorelaxation effect of any CFRs fraction, whether polar or non-polar. The aim of this study was to compare the bronchorelaxation effect of MECFR with CFRs hexane (non-polar) fraction, CFRs ethyl acetate (semi-polar) fraction, and CFRs methanol (polar) fraction. The study was conducted using the isolated bronchial ring of guinea pigs [8]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Asian Journal of Pharmaceutical and Clinical Research
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
