In vitro study of solubilization ability of bovine pulp tissue using different irrigating solutions

Abstract

The aim of this study was to evaluate in vitro the capacity of solubilization of bovine pulp tissue, promoted by the following auxiliary chemical solutions: 2.5% sodium hypochlorite at pH 9, 5.25 % sodium hypochlorite at pH 9, 2% chlorhexidine gel at pH 6, 17% EDTA at pH 7, and Smear-Clear®. Material and methods:A total of ten specimens of bovine pulp tissue, weighing 1.65 g each, were placed into flasks connected to a device developed for the study in order to reproduce irrigation dynamics. The flasks with the specimens received a volume of 80 ml of irrigating solution and the experimental times were 15, 30, 45 and 60 minutes. Results: The results were analyzed statistically by Kruskal-Wallis test to compare the different experiment times (15, 30, 45 and 60 minutes) of each irrigating solution. To compare the variation among the times of one solution, Kolmogorov-Smirlov test (Lilliefors) was used. Conclusion:Considering the results obtained and the limitations of the study, it can be concluded that 5.25% sodium hypochlorite exhibited the greatest solubilization ability, followed by 2.5% sodium hypochlorite, Smear-Clear®, 17% EDTA and 2% chlorhexidine gel.