In Vitro study of green synthesized ZnO nanoparticles on human lung cancer cell lines

Abstract

This paper presents green synthesis of ZnO nanoparticle (L1-NPs) using extract of leaves of Azadirachta indica (Neem tree) Characterizations of synthesized L1-NPs have been done by UV–Visible spectroscopy, FTIR, powder x-ray diffraction (PXRD), Scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), field emission scanning electron microscopy (FE-SEM). The effect of synthesized nanoparticles on viability of human lung adenocarcinoma (A549 cells) have been determined using MTT assay. The viability of A549 cells have been significantly decreased by ZnO NPs synthesized with leaves extract as well as only extract (OE) of leaves of Azadirachta indica (Neem tree) in a dose-dependent manner. In addition, we have observed that the cell viability could be significantly reduced with an incubation period of 48 h at 100, 150,200 and 250 µg/mL. The IC50 value for zinc oxide nanoparticles prepared with 1 mL of leaves extract (L1-NPs) has been obtained 138.50 μg/ml after 48 h incubation.