In vitro study of antioxidant, antidiabetic and antiurolithiatic activity of synthesized silver nanoparticles using stem bark extracts of Hybanthus enneaspermus

Abstract

Diabetes mellitus is an endocrine disorder that affects 85% of the people because of higher sugar level in blood. Due to this metabolic disorder and improper function of organs especially kidney results in urinary tract infection causing struvite urinary stone. So, in the present study was carried out to study the green synthesized silver nanoparticles using Hybanthus enneaspermus stem bark extracts on antioxidant, antidiabetic and antiurolithiatic activity under in vitro conditions. By the treatment of aqueous solution of 5 mM silver nitrate (AgNO3) with stem bark extracts, silver nanoparticles could be quickly synthesized within 1 h. These silver nanoparticles were characterized using UV–Visible spectroscopy, Fourier Transform Infrared Spectroscopy (FT-IR), Transmission Electron Microscope (TEM), X-ray diffraction (XRD) and Dynamic Light Scattering (DLS) for further confirmation. TEM analysis found that the silver nanoparticles are spherical in shape. X-ray diffraction confirmed that silver nanostructure exhibit a face centered cubic crystal structure. DLS showed that the nanoparticles size is 644.2 nm. By increasing the concentration of silver nanoparticles, weight of the formed crystals reduced from 0.94 g to 0.13 g in struvite crystals and analysed by FTIR analyses. This multidisciplinary approach showed a better percentage of inhibition such as antioxidant, antidiabetic and antiurolithiatic activity of silver nanoparticles.