Abstract
Purpose: Purpose: To evaluate the autophage and neuroprotective effects of WGM, a Chinese traditional medicinal prescription in a Parkinson’s disease mouse model. Methods: Methods: Cell viability was measuredcusing a LHD-release assay and MTT assay. To find out the autophagyrelative mRNA gene expression in BV2 microglia cell line, LC3, LC3a, LC3b, Beclin-1, ATG5 were examined by RT-PCR. To find out the autophagy-relative protein expression of signal events in BV2 microglia cell line, LC3b-1, LC3b-II, Beclin1 were examined by western blotting. To find out the autophagy detection in BV2 microglia cell line. Cells were assayed for autophagy positive cells by FACS analysis. To measure the amount of dopamine in mice brain, ST-SNpc were examined by Bradford. Immunohistochemistry was examined in the MPTP-induced Parkinson’s disease mice to evaluate the neuroprotective effects of WGM on ST and SNpc. Ischemic mice brain stainedwith TTC in theMPTPinduced Parkinson’s disease to find out ischemia in mouse In order to investigate the effect of WGM on recovery of memory, we examined the memory by usingMorris watermaze test. Immunohistochemistry was examined in the MPTP-induced Parkinson’s disease mice to evaluate the neuroprotective effects of WGM on hippocampal lesion. In order to investigate the effect of WGM on recovery of behavioral deficits, we examined the motor function by using and FST. The convenient, simple, and accurate HPLC method was established for simultaneous determination of Neurotransmitters in MPTP-WGM group. Results: Results and Conclusion: WGM can enhance the induction of autophagy through key regulator beclin1 and LC3b-II. WGM inhalation in MPTP mice led to the restoration of behavioral impairment and rescued dopamine, TH-IR cell, hippocampus, serotonin. Furthermore,WGMessential oilmay serve as a potential preventive or therapeutic agent regarding Parkinson’s disease. Conclusion: Acknowledgement: This research was supported by Basic Science Research Program through the NRF funded by the Ministry of Education, Science and Technology(201401850001) Contact: Taek-Won Ahn, twahn@dju.kr
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