IN VITRO STUDIES ON THE PROTECTIVE EFFECT OF TANNIC ACID OF U87 CELLS INDUCED BY BETA-AMYLOID

Abstract

This study shows that tannic acid is an Alzheimer's disease (AD) model cell line on amyloid-beta (Aβ)-induced U87 (human astrocytoma cell line) in β-amyloid (Aβ)-induced in vitro Alzheimer's Disease (AD) model. We aimed to investigate the effects of antioxidants.In the study; Three groups were formed as the control group, the Aβ group, and the Aβ + tannic acid group obtained by adding tannic acid to the Aβ group. Firstly, the cytotoxic potential of TA in U87 cells was investigated by the colorimetric MTT (3-4,5-dimethyl-thiazolyl-2,5 diphenyltetrazolium bromide) test. To determine the antioxidant status in the cell line treated with Thymoquinone, to examine the effects of superoxide dismutase (SOD), catalase (CAT) activities, total oxidant status (TOS), and total antioxidant status (TAS) were measured by the ELISA method. When compared to the control group, the SOD, CAT, and TOS levels were significantly decreased in the U87 cell line exposed to Aβ; TAS levels were found to increase significantly. However, the application of tannic acid to the Aβ-U87 cell line significantly increased SOD, CAT, and TAS levels; It was found that it decreased the TOS level. In in vitro experiments, we determined that tannic acid has a protective effect by increasing antioxidant parameters in the amyloid beta-induced cell line.