Abstract
An in vitro model for studies of preovulatory follicle and ovulation is described, using a recirculating system for perfusion of isolated rabbit ovaries. The preovulatory follicle was studied by addition of human chorionic gonadotropin (hCG) to the perfusion medium 1.5 h after the start of perfusion. Ovulations occurred approximately 13 h after in vivo hCG injection to the animal and 5-10 h after the start of perfusion. The ultrastructural changes during the perfusion were similar to in vivo conditions. The release of estradiol and progesterone into the medium are consistent with in vivo measurements. This in vitro model is probably useful for further studies of the biochemistry and morphology of ovarian function.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
More From: Research in experimental medicine. Zeitschrift fur die gesamte experimentelle Medizin einschliesslich experimenteller Chirurgie
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.