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Abstract
Root development was obtained from isolated embryos of Pinus pinea in the presence of NAA and activated charcoal. The concentration of the hormone strongly influenced the frequency of root formation. Furthermore, the concentrations of the culture media (salts and vitamins) and of BAP markedly influenced the frequency of bud formation, giving the highest value (67.8±2.4) to the basal medium to which 5 μmol BAP was added. Bud induction in embryos of Pinus pinea was also affected by temperature. Bud formation was observed after 6 days at 26 °C, but at 20 °C it was first observed after 17 days.
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