In vitro storage of sweet passion fruit seeds as an innovation conservation alternative

Tatiana G Junghans,Onildo N Jesus,Michele S Ferreira,Jailton J Silva

doi:10.1590/0001-3765202120190901

Tatiana G Junghans, Onildo N Jesus + Show 2 more

Open Access

https://doi.org/10.1590/0001-3765202120190901

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Abstract

Abstract Sweet passion fruit (Passiflora alata Curtis) is a species native to Brazil that is economically important for fruit consumption and can also be used as an ornamental plant. The objective of this work was to evaluate different forms of storing Passiflora alata seeds for the purpose of preserving accessions in germplasm banks. Three experiments were performed. In the first, the seeds without aril were stored for two periods (one and three years) in plastic bags and in in vitro culture medium at two temperatures. In the second, the seeds with and without aril were stored for one year in plastic boxes and in vitro culture medium at two temperatures. In the third, the seeds were kept for two years in different in vitro culture media. The seeds quickly lost viability when stored for one year conventionally in a cold chamber with 25% emergence, but remained viable when were stored in vitro with 90% emergence. Therefore, the use of in vitro culture medium is an innovative way to preserve sweet passion fruit seeds at 25 oC. The seeds of the Passiflora alata evaluated should be considered recalcitrant.

Highlights

Sweet passion fruit (Passiflora alata Curtis) is a species native of the Amazon region of Brazil, Peru and Colombia that is economically important for fruit consumption and can be used as an ornamental plant (Figure 1)
Plant material and culture medium The experiments were conducted in the Tissue Culture Laboratory, the Seed Conservation and Technology Laboratory and a greenhouse of the Empresa Brasileira de Pesquisa Agropecuária (Embrapa) Cassava and Fruits research unit (Embrapa Mandioca e Fruticultura), in the municipality of Cruz das Almas, Bahia (12° 39’ 25” S, 39° 07’ 27” W, 226 m)
The P. alata seeds were obtained from fullyripe fruits harvested from the experimental field of the Active Germplasm Bank of Passiflora of Embrapa Cassava and Fruits

Summary

Introduction

Sweet passion fruit (Passiflora alata Curtis) is a species native of the Amazon region of Brazil, Peru and Colombia that is economically important for fruit consumption and can be used as an ornamental plant (Figure 1). The leaves have sedative and tranquilizing properties (Provensi et al 2008, Klein et al 2014) and are a good source of antioxidant, anti-inflammatory and potentially of anti-diabetic compounds (Colomeu et al 2014, Figueiredo et al 2016). It has wide distribution in Brazil, from the states of Amazonas in the north to Rio Grande do Sul in the south (Bernacci et al 2015). It is fundamental to know the germinative potential and conservation capacity of its seeds, to improve the production of seedlings and the management of germplasm banks

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