Abstract

Leucocytes from peripheral blood (PBL), spleen and head kidney of Atlantic salmon ( Salmo salar ) and rainbow trout ( Oncorhynchus mykiss ) responded well when stimulated with lipopolysaccharide from Escherichia coli and phytohaemagglutinin (PHA). Thymocytes responded to PHA only. PBL from Atlantic salmon vaccinated against furunculosis showed significantly higher responses as compared to non-vaccinated fish when stimulated with an antigen preparation of whole cells of Aeromonas salmonicida . In leucocyte cultures from rainbow trout vaccinated against furunculosis, specific antibodies to A. salmonicida were produced in vitro . None or very low antibody levels were detected in cultures from non-immunised fish during the first 7 days in culture. After 9 days, a primary antibody response to A. salmonicida could, however, be detected in these cultures. Good correlation ( r =0·88) between antibody production by blood leucocytes and splenocytes was seen.

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