Abstract
A microculture system is described to study the in vitro properties of murine spleen cells utilizing as few a 6 × 10 5 cells per culture. The use of a new multiple automated sample harvester enabled rapid multiple tests using relatively few cells. This new procedure was highly reproducible and diminished the source of many errors. Data are presented to show the many variables that are involved in studying the PHA and PWM responses of murine spleen cells in this microculture system. The procedure provides a relatively simple technique for studying the various in vitro effects of specific and non-specific stimulation of leukocytes especially when very few cells are obtainable or in small animals where pooling of the cells lead to masking individual variations.
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