In vitro spermatogenesis of shark minnow fish (Osteochilus hasselti Valenciennes 1842) as a potential fish reproductive biotechnology

Abstract

In vitro spermatogenesis has many clinical applications and hopefully with improvement of research findings this technique will solve the applied fisheries era 4.0. This research aimed to evaluate the effects of testosterone and type of serum on the in vitro spermatogenesis of shark-minnow fish (Osteochilus hasselti). In the present research, four concentrations of testosterone (0, 5, 10 and 15 ng/mL) were tested. The types of serum used were autologous serum and Fetal Bovine Serum (FBS). The results showed that the use autologous serum in the culture medium capable of maintaining the consistency of the tissue culture in culture period and maintaining in vitro spermatogenesis of shark-minnow. This study shown that stage-specific of spermatogenic cells needed certain testosterone concentration. The stage of secondary spermatocyte needed testosterone concentration of 5ng/mL i.e. 50.81±9.29% in medium with autologous serum and 36.47±15.49% in medium with FBS, while stage of spermatid and spermatozoa needed testosterone concentration of 10ng/mL i.e. 36.66±19.81% and spermatozoa 43.45±23.44% in autologous serum and the proportion of spermatid i.e. 42.35±9.09% and spermatozoa 35.25±14.0% in medium with FBS. This study proven that in-vitro spermatogenesis, already demonstrated in shark-minnow fish, offered great promises to cope with reproductive issues in the aquaculture and applied fisheries biotechnology.