Abstract
Blocks in chlorophyll (Chl) biosynthesis in crude extracts of well-defined chlorophyll-deficient seedlings and green siblings from five Pennisetum glaucum (L.) R.Br. genotypes and the ability of these genotypes to relieve such blocks in crude extract mixtures were studied with low temperature (77 K) fluorescence spectroscopy. Dark-adapted green seedling extracts exhibited F657, F685, and F735 peaks corresponding with protochlorophyllide (Pchlide), Chl from photosystem II and from photosystem I, respectively. Extracts of white seedlings from white stripe mutants and an albino mutant exhibited F657 peaks indicative of Pchlide, whereas the extracts of yellow seedlings from yellow stripe mutants exhibited F657 and F678 peaks representing Pchlide and chlorophyllide (Chlide) accumulation, respectively. Addition of their green sibling's extract resulted in the appearance of F685 and F735 peaks, suggesting de novo relief of the blocks and photoreduction of Pchlide to Chl(ide). Incubating a mixture of crude extrac...
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