Abstract
Abstract Slowly growing calli were induced in vitro from nucellar explants excised from fertilized ovules of immature 4.0-4.8 cm long monoembryonic mango fruitlets. The medium consisted of Murashige and Skoog formulation that had been modified accordingly: half strength major salts, 60 g/liter sucrose, 400 mg/liter glutamine, 100 mg/liter ascorbic acid, 1.0 mg/liter 2,4-dichlorophenoxyacetic acid (2,4-D), and 8 g/liter Difco Bacto agar. Somatic embryogenesis occurred from callus on this medium and following subculture to medium without growth regulators. Maturation and limited germination of somatic embryos occurred in the absence of growth regulators.
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