Abstract
The effect of zeatin, NAA (α-naphthaleneacetic acid), putrescine and cefotaxime on the frequency of shoot regeneration from Betula pendula ‘Dalecarlica’ EM 85 leaf discs has been examined. About 80% of leaf discs were induced to form adventitious shoots when the culture medium contained 45.6 μmoll-1 zeatin and 0.1 mmoll-1 cefotaxime. The addition of NAA to zeatin-containing media prevented shoot regeneration but stimulated root development directly from leaf tissues. Putrescine (0.1 mmoll-1) and cefotaxime (0.1 mmoll-1) could both significantly increase the percentage of leaf discs regenerating on optimal zeatin-containing media, and increase the number of shoots per regenerating disc.
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