Abstract
Chili pepper (Capsicum annuum L.) is an economically important spice crop in tropical and subtropical countries. In vitro plant regeneration was obtained from 15 th day old hypocotyl explants of three chili pepper cultivars (Capsicum annuum L., var. ‘X-235’, var. ‘PC-1’ and var. ‘Pusa Jwala’). Among the genotypes of Capsicum L. var. ‘X-235’ responded better than the var. ‘PC-1’ and var. ‘Pusa Jwala’. MS medium containing BAP (4.0 mg/l) and IAA (0.5 mg/l) was found to be the best medium for the production of maximum number of shoot buds in all the genotypes of chili pepper i.e., 6.80±0.16 (var. ‘X-235’), 5.00±0.19 (var. ‘PC-1’) and 4.80±0.12 (var. ‘Pusa Jwala’). The shoots were rooted on MS medium fortified with IBA (0.5 mg/l). Rooted plants were hardened and transplanted to the soil. The plants showed 80-90% survival during transplantation.
Highlights
Capsicum annuum L. is an economically important crop plant belonging to the family Solanaceae; two main consumption types of pepper- spice and vegetable are prevalent throughout the world
The results are in conformity with the findings of Gunay and Rao (1978); Christopher and Rajam (1996); Ramirez-Malagon and Ochoa-Alejo (1996); Sanatombi and Sharma (2008) that reveals the regeneration of plants from hypocotyl explants in diverse cultivars of Capsicum L
The shoots derived from hypocotyl explants were excised and implanted on MS medium supplemented with IAA and IBA in three different concentrations (Tab. 2)
Summary
Capsicum annuum L. is an economically important crop plant belonging to the family Solanaceae; two main consumption types of pepper- spice and vegetable are prevalent throughout the world. The regeneration capacity of chili plants has been carried out successfully from hypocotyl explants (Borychowski et al, 2002; Christopher and Rajam, 1994, 1996; Fari and Czako, 1981; Gunay and Rao, 1978; Mok and Norzulaani, 2007; Rodeva et al, 2006; Sanatombi and Sharma, 2008; Singh and Shukla, 2001) Many of these reports suggest a strong influence of the genotype on the regeneration process (Christopher and Rajam, 1996; Ochoa-Alejo and Ireta-Moreno, 1990; Ramirez-Malagon and Ochoa-Alejo, 1996; Szasz et al, 1995). The study was undertaken to determine the regeneration potential and to develop efficient in vitro plant regeneration protocol for the three genotypes of C. annuum L
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