Abstract
Isolation of the consensus sequence! This was the surprising result when in vitro selection was applied to the hammerhead ribozyme to obtain variants with higher cleavage rates at the 3′ side of some NUH triplets, such as GUC and AUA, and of GAC triplets. This indicates that the wild-type sequence (see picture) has optimally evolved for catalysing these reactions. An exception was the identification of a ribozyme cleaving at the 3′ side of AUG, which is not hydrolysed by the consensus ribozyme. This ribozyme variant contains considerable changes in the core and the stem–loop II regions and is thus hammerhead-like.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Chembiochem : a European journal of chemical biology
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
