In vitro selection and characterization of Ni-tolerant callus lines of Setaria italica L

Abstract

Nickel tolerant callus lines of Setaria italica L. were developed from callus cultures grown on MS medium supplemented with 0.5 mg·dm−3 kinetin+2.0 mg·dm−3 2,4-D+2.0 mg·dm−3 Ni+2. Standard growth parameters such as callus fresh and dry weight, growth tolerance index were used as indicators of nickel toxicity. Measurements as early as 2 weeks after the beginning of the treatments did not yield consistent results. However, growth tolerance index at 4, and 8 weeks after the beginning of treatments yielded significant differences among the non-tolerant and tolerant calli. The tolerant calli has enhanced growth at 2.0 mg·dm−3 Ni+2 while non-tolerant calli showed a reverse trend in growth in the presence of 2.0–2.5 mg·dm−3 of nickel. The tolerant calli differentiated into mass of embryogenic calli within 4 weeks of culture which could be maintained for prolonged period without loss of regenerative capacity.