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Abstract
Abstract Hibiscus coddii subsp. barnardii is an endemic South African plant species with ornamental potential. The limited plant availability in nature and restrictions on the collection of living plant material (cuttings) for cultivation purposes, necessitated the development of a protocol for in vitro seed propagation to ensure all year round ex vitro establishment of plants. The effect of culture medium on seed germination and seedling performance was therefore studied. Various types of sterile seed cultures, namely moist filter paper bridges, five different strengths (¼, ½, –, full, 1¼) of PGR-free Murashige and Skoog medium (MS) and solid Gelrite® medium (control) were investigated. High germination percentages (85%-98%) were found in all three seed cultures, although seeds germinated on filter paper, Gelrite ® and lower strengths (¼, ½, –) of MS medium had shorter mean germination times (1.6-2.8 days) than those germinated on full and 1¼ strengths (3.5-4 days) of MS medium. Furthermore, the growth performance of seedlings varied with the strength of MS medium and was best supported on full strength MS medium, whereas ¼ and ½ strength MS media and Gelrite® cultures were detrimental for seedling growth.
Highlights
Hibiscus coddii Exell subsp. barnardii (Exell) Leistner & P.J.D
The method used for surface disinfection of chemically scarified seeds of H. coddii subsp. barnardii proved effective for establishment of aseptic conditions for in vitro germination in all seed cultures since no microbial contaminations were observed
Significant differences were observed among the different strengths of Murashige and Skoog (MS) media and the Gelrite® cultures with respect to the mean germination time (MGT) and the germination rate index (GRI) (Table 1)
Summary
Barnardii (Exell) Leistner & P.J.D. Winter (family Malvaceae) is an endemic South African flowering plant species with the commercial potential to be grown as an ornamental pot plant or in rockeries in drought-prone areas (Craib, 2003). Successful propagation and multiplication of this plant are, imperative for its introduction into the horticultural market. Propagation of ornamental plants is mainly by means of seeds, cuttings, and in vitro culture (Davies et al, 2017). Collection of living plant material (cuttings) from wild. Barnardii plants for propagation purposes is, restricted. This necessitated the development of a protocol for seed propagation to generate stock plants
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