In vitro seed germination and protocorm development of Epidendrum catillusRchb. F & Warsz. (Orchideaceae), in different culture media

Abstract

The application of techniques is important for the massive and efficient propagation of orchids, without affecting the natural environment and its conservation.The objective was to evaluate the effects of six culture media: t1, Knudson; t2, MS 75% + NAA (1,0 mgL-1) + GA3 (3,0 mgL-1); t3, MS 75% + 100 mLL-1coconut water; t4, MS 100% + GA3 (3,0 mgL-1); t5, MS 100% + NAA (1,0 mgL-1) + GA3 (5,0 mgL-1); t6, MS 100% + 100 mLL-1coconut water on in vitro seed germination and protocorms formation of the orchid E. catillus. Seeds were obtained from a capsule in stage of physiological maturity from sector Grapanazú, Huancabamba, province of Oxapampa-Pasco, Peru. A randomized complete block design was used. It was found that, at 64 and 71 days, the treatments t1, t3, t4 and t6 had the same seed germination and development of in vitro protocorms, however, all of them were higher than t2 y t5. We concluded that Knudson media, MS 75% and 100% + 100 mLL-1coconut water and MS 100% + GA3 (3,0 mgL-1), enhanced seed germination.