In vitro screening of plants from the Brazilian Caatinga biome for methanogenic potential in ruminant nutrition.

Abstract

Thirty-nine plants naturally found in Brazilian Caatinga semiarid biome were screened using an in vitro fermentability testing focused in apparent organic matter digestibility (aOMD), gas, methane (CH4), and short-chain fatty acid (SCFA) production. Three independent in vitro runs were carried out and plants were classified by CH4 concentration as proportion of gas and per unit of apparent digested organic matter (aDOM). According to its CH4 concentration on produced gas (mL/L), the plants were classified as low (> 110), medium (from 60 to 110), and high (< 60) anti-methanogenic potential. From evaluated plants, 3, 24, and 12 were classified as high, medium, and low anti-methanogenic potential. High anti-methanogenic potential plants Cnidoscolus phillacanthus (CnPh), Chloroleucon foliolosum (ChFo), and Anadenanthera macrocarpa (AnMa) produced 21.3, 34.3, and 35.9mL CH4/L of gas. Methane concentration for Myracrodruon urundeuva (MyUr) was 61.1mL/L and classified as medium potential. However, CH4 production per unit of aDOM was similar between MyUr and AnMa (3.35 and 2.68mL/g, respectively). Molar proportions of acetate and propionate in SCFA produced by ChFo fermentation were 0.02 and 0.78mmol/mol. Acetate to propionate ratios were 0.79, 0.03, 1.39, and 1.36 for CnPh, ChFo, AnMa, and MyUr, respectively. Greater aOMD were observed for Opuntia sp. and Calotropis procera (632 and 601g/kg, respectively), which were classified as medium mitigating potential plants. AnMa, ChFo, CnPh, and MyUr are promising anti-methanogenic plants for ruminants. Selecting forages to feed ruminants in Caatinga is a potential strategy for enteric CH4 emission reduction, and our in vitro results can support future research by indicating species to be evaluated in in vivo studies integrating mixed diets with performance, digestibility, and CH4 production, yield, and intensity. Graphical abstract ᅟ.