In vitro screening of ninety-five plant extracts for inhibition of Clostridium histolyticum collagenase and Bacillus polymyxa metalloprotease- analogous human MMPs

Abstract

Introduction and Aim: Matrix metalloproteinases (MMPs) are involved in many human diseases. Medicinal plants and their different constituents are utilized as therapeutic agents. In the present work we report the total phenolics content and screening of ninety five plant extracts for inhibitory activities against Clostridium histolyticum collagenase (ChC) and Bacillus polymyxa metalloprotease (BpM). Materials and Methods: Total phenolics content from plant methanolic extracts were assessed using Folin-Ciocalteau assay. Screening of plant extracts for ChC and BpM inhibitory activities was performed using dot blot assay on X-ray film. Results: G. superba fruit was observed to have the highest phenolics (257.58 ± 0.75 mg GAE /g tissue) content while C. nurvala stem was observed to have the lowest phenolics (0.468 ± 0.003 mg GAE /g tissue) content. Thirty two plants showed ChC inhibitory activities while twenty seven plants showed BpM inhibitory activities. From those plants twenty five plants have common ChC and BpM inhibitory activities. Most of plant barks were found to have a high amount of total phenolics as compared to other plant tissues while the most of plant leaves were found to have low amounts of total phenolics. Most of barks were found to exhibit inhibitory activities as compared to other tissue. Only 6 different leaves were found to exhibit inhibitory activity out of 42 different leaves. None of the root extracts exhibited inhibitory activities. Conclusion: The inhibitory activity exhibiting plant extracts could be used as a significant source for the determination of novel drug candidates to treat MMPs related diseases.