Abstract
The present study purpose is to evaluate the potentiality of the Deproteinised juice made from selected plants to induce the enzyme productivity by growing the fungi on it. It is because, in earlier findings, the DPJ was found potential in enhancing fungi and the plant growth when used as the medium. The internal factors are responsible in DPJ which induces plants and fungi growth. During the process of Green Crop Fractionation (GCF), the deproteinised (DPJ) obtained from tissues of cabbage, beet, lucerne (Alfalfa), carrot and Anathum (Dill) forages left after leaf protein extraction employed as a medium for the cultivation of mycelial biomass of Penicillium and Aspergillus fungi. The mycelial growth in vitro was compared with the glucose nitrate medium. The culture filtrates were used to screen different secreted hydrolytic or cell wall degrading enzymes.. The agar ‘cup‐plate’ diffusion technique has been applied to the quantitative determination of enzyme activity, principally to amylase, cellulase and protease. With all enzymes so far examined, the relationship between diameter of zone over a wide range secreted quantitatively was examined. All fungi grew well on DPJ in comparison to their growth on glucose nitrate (GN) medium. Comparatively with GN medium, lucerne DPJ was found having more mycelial cellular proliferation. When the fungi grown on different concentrations of substrates, enriched with carboxymethyl cellulose, casein and starch in deproteinised leaf extracts and GN medium, it was found that there was the enhancement in the mycelial dry weight grown on DPJ as compared with glucose nitrate medium. Penicillium showed more yield of enzyme activities especially of cellulases and amylases as compared to protease by cup plate method. There was enhancement of mycelial biomass when the concentrations of substrates increased from 1% to 2%, while there was no change in the activity of all enzymes by increasing the concentrations of substrates. Activities of the enzymes in vitro can be indicative of the pattern of organogenesis in callus cultures in further studies by fungal culture filtrates cultured on deproteinised fluid medium.
Highlights
In view of using plant nutrients more efficiently, Prof
The leaf protein concentrate (LPC) is isolated from remaining portion of leaf juice, called as deproteinised juice (DPJ), by filtration through cotton cloth.The juice liberated consists of enzyme proteases and amylases
The use of LPC as a protein and vitamin A supplement in human and animal nutrition is well recommended while the DPJ is considered as a byproduct of this process (Pirie 1987)
Summary
In view of using plant nutrients more efficiently, Prof. Pirie (1942) advocated the process of green crop Fractionation (GCF). The LPC is isolated from remaining portion of leaf juice, called as deproteinised juice (DPJ), by filtration through cotton cloth.The juice liberated consists of enzyme proteases and amylases. When this juice was fermented for enhancing hours, it breaksdown the protein and carbohydrates, estimated by cup plate diffusion assay. The use of LPC as a protein and vitamin A supplement in human and animal nutrition is well recommended while the DPJ is considered as a byproduct of this process (Pirie 1987). While on the other hand, DPJ from Amaranthus enhanced enzymes proteases and cellulases from the culture filtrates of Trichoderma. When It is used at high concentrations, it caused phytotoxicity in Pea plants during growth. These enzymes proved to be better, cheaper and environment friendly compared to the use of chemicals
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