Abstract
Rooting induction is a very important stage in in-vitro for the anticipation of the extinction, In this paper, the rooting induction of embryo somatic dendrobium species from Riau Province Indonesia is studied using different sucrose concentrations (0 g L-1; 25 g L-1; 50 g L-1; 75 g L-1), and Kinetin (0 mg L-1; 0,1 mg L-1; 1,0 mg L-1; 10 mg L-1), in a modified MS medium containing half the regular concentration of macronutrients at pH 5.6, with 50 g L-1 sucrose and 1,0 mg L-1 kinetin was optimal for a percentage of explant producing root, a number of roots, root high, root and shoot ratio (T/R Ratio).
Highlights
Orchid species is an orchid plant that grows on an area and has not undergone a genetic engineering process, This plant generally grows and develops with a compact color, and does not have many floral patterns, such as white, or red
Earlier research on the induction of rooting especially in the Dendrobium orchid plant species has not been found, but the research on the use of kinetin on embryo growth that examines kinetin on the growth of embryonic shoots have been done by Heriansyah, 2019
This study aims to study the induction techniques of rooting on the embryo somatic orchid plant species, For the conservation of locally endangered orchids due to various activities that interfere with the preservation of habitat from the Dendrobium orchids
Summary
Orchid species is an orchid plant that grows on an area and has not undergone a genetic engineering process, This plant generally grows and develops with a compact color, and does not have many floral patterns, such as white, or red. The planting of Dendrobium orchid plants in-vitro cultures has problems due to the induction of rooting which requires a relatively long time, After the establishment of the callus and multiplication process so it is necessary to provide induction of rooting treatment. Earlier research on the induction of rooting especially in the Dendrobium orchid plant species has not been found, but the research on the use of kinetin on embryo growth that examines kinetin on the growth of embryonic shoots have been done by Heriansyah, 2019. Explaining that the administration of kinetin affects the establishment of a dendrobium of explants species (Heriansyah 2019)
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