Abstract
In production, shoot cuttings of lavender are most often used for vegetative propagation, however, this method does not promote high propagation rate. The most effective method is propagation by axillary or terminal buds that requires further plant rooting in vitro. Due to, objective of our work was analysis of lavender in vitro rhizogenesis. Investigation was performed on Lavandula angustifolia cultivars with different concentration of the growth regulators in medium. After18 Day, Only 1 mg/l NAA did not stimulate rhizogenesis for ‘Record’ and ‘Sineva’ cultivars. Root primordia formed from the cambium cells. Further root growth accompanied by cleavage of the core tissues and they appeared on the shoot surface. Morphologically, a root cap, meristem, elongation zone and zone with root hairs were identified in de novo formed root in vitro. Along with normally roots, the appearance of roots accreted along their periphery with free apexes and roots with the decreased meristem was also noted. For ‘Prima’ cultivar, highest values of the mitotic index were observed on the hormone-free half-strength MS medium and on ½MS medium with 1.0 mg/l NAA. Thus, our data showed that root morphogenesis for lavender cultivars depended on the plant material and culture medium.
Highlights
In vitro tissue culture method allows to obtain plants from individual cells, tissues and organs on culture media
Vegetative propagation of lavender is most often used by shoot cuttings, this method does not promote high propagation rate [8, 9]
The first phase corresponds to the cell differentiation; the second is sensitive to organogenic factors, such as growth regulators, which determine the formation of individual organs and are critical during this period; third is the deployment of a differentiation program [16]
Summary
In vitro tissue culture method allows to obtain plants from individual cells, tissues and organs on culture media. Morphogenesis is a complex process and its regulation is carried out at the cellular, tissue and organismal levels by factors such as light intensity, temperature, gas environment [1], nutrient composition, type of explant, its orientation on the medium [2, 3] and internal hormonal balance of explant [4] These all determine the processes of division, elongation, differentiation, aging and cell death [5]. There is necessity for fast and more effective rooting method in vitro for lavender plants. Objective of this investigation was to study rhizogenesis for lavender on different growth culture media
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