Abstract
The effectiveness of different elicitation variants in combination with alarmone application was studied in shoot cultures of Polyscias filicifolia. The shoots were elicited with 200 µM methyl jasmonate (MeJA) or 50 µM salicylic acid (SA) alone or in combination, and their activity was compared with those treated with the alarmone diadenosine 5′,5‴-P1P3-triphosphate (Ap3A), either alone or in combination with SA and/or MeJA. All treatments resulted in significant stimulation of phenolic acid production (chlorogenic and ferulic acids), as well as oleanolic acid (OA) compared to control, with their highest concentration noted under simultaneous elicitation with SA and MeJA. While the maximum content of caffeic acid was detected after treatment with alarmone alone. In each of the culture variants enhanced antioxidant activity was observed, however the level varied according to the treatment. In addition, the SA, Ap3A and Ap3A+SA variants demonstrated additional peroxidase isoforms, as indicated by Native-PAGE, as well as the highest α-tocopherol content. The highest antioxidant capacity of shoot extracts was correlated with the highest abundance of phenolic compounds and OA. The results indicate that ROS induction appears to participate in the signal transduction following Ap3A treatment.
Highlights
IntroductionPlant secondary metabolites are a wide and diversified group of low molecular weight compounds
Published: 17 February 2021Plant secondary metabolites are a wide and diversified group of low molecular weight compounds
The highest shoot multiplication rate (3.17 ± 0.08) was achieved for shoots of P. filicifolia grow on LS medium with the addition of Ap3A, this result was statistically significant only in comparison to the effect of methyl jasmonate (MeJA), where the proliferation rate amounted to 2.05 ± 0.05 and was the lowest among all tested combinations
Summary
Plant secondary metabolites are a wide and diversified group of low molecular weight compounds They are known to act as biotic and abiotic stressors and play a crucial role in the responses of plants to changing environmental conditions. The negative cross-talk between SA and JA pathways was reported [4,5], this relation could be shifted from antagonistic to synergistic depending on the concentrations of SA and JA. Such flexibility would enhance the potential for the plant to adapt to a wider range of environmental stimuli [2,5]. The plant defence system, which is launched in response to excessive toxic ROS levels, consists of enzymatic antioxidants such as catalase
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