Abstract
ABSTRACTThe in vitro response of anther cultures of 19 Bulgarian pepper (Capsicum annuum L.) genotypes among which eight lines, seven varieties and four hybrids was examined using two induction media (C and Cm) within two time intervals for cultivation—12 and 40 days respectively, and two regeneration media (R and Rm). Six lines, six varieties and three hybrids produced direct embryos in the cultivated anthers 35–40 days after inoculation in vitro. Regenerated plants were obtained from four lines, six varieties and one hybrid. It was established that 12 days of cultivation on medium C were not sufficient to initiate embryo formation in the tested genotypes while the longer one (40 days) revealed their ability for direct embryogenesis. Considerable percentage of embryos developed into plant-regenerants after being transferred on R medium (50-100%). Higher frequency of embryogenic anthers was recorded after 12 days of culturing on induction medium Cm but after their transfer on Rm medium the embryos did not develop into plantlets. These results suggest that the donor genotypes have specific requirements for nutrient medium and duration of induction cultivation.
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