In vitro Research of the Concentration Dependence of Effect of Adrenaline on Platelets Aggregation.

Abstract

Purpose. Paper intended to present experimental evidences that adrenaline has a direct effect of inducing platelets aggregation in the concentration range 1-8µM.Material/Methods. Platelet rich plasma from patients of Colentina Clinical Hospital, following an informed consent. The platelet rich plasma (PRP) was prepared by centrifuging the anticoagulated sample at 200 G for 10 minutes. Aggregation was evaluated by optical aggregometry, classical method of Born, using Helena PACKS-4 Aggregometer.Results. The curves transmission light-time followed the structure: a lag-time, a first phase aggregation, more or less linear, defined by a “Slope 1”, a second wave of aggregation defined by “slope 2” and a “saturation” phase. Slope 1 increases with the concentration of adrenaline. The second slopes of the aggregation curves, maximum aggregation and areas under curves depended linear on adrenaline concentration. Conclusions.Adrenaline, in concentrations in the 1-8µM, induce aggregation of human platelets from platelet rich plasma. Linear regression models for slope and area were practically identical suggesting a rather unique than biphasic mechanism of action of adrenaline during the time course of aggregation.