In vitro regeneration of Psoralea corylifolia L. through callus cultures

Abstract

In vitro regeneration protocol was developed from hypocotyl-derived callus of Psoralea corylifolia L. Green compact nodular calli were induced from 3 day-old hypocotyl explants on Phillips and Collins (L2) medium containing 25 g l−1 sucrose, 7 g l−1 agar and supplemented with 10 μM NAA and 2 μM TDZ. Higher shoot regeneration (89.5 ± 1.18) was achieved in enriched L2 medium supplemented with 2 μM BA, 4 μM TDZ and 50 mg l−1 BVN. Regenerated shoots elongated on 1/2-enriched L2 medium containing 10 g l−1 sucrose, 7 g l−1 agar and 2 μM 2iP. Elongated shoots (45–55 mm in length) were exposed simultaneously 15.0 roots per shoot as well as hardened in moistened (1/8-L2 basal salt solution with 5 mM IBA and 100 mg l−1 BVN) soil mixture and vermiculite (3 : 1 v/v). The plants were subsequently established in the field. The higher survival percentage (100%) was achieved in winter season (September–December, 25–28°C). This system would be useful for mass propagation and germplasm conservation of P. corylifolia.