Abstract
In vitro regeneration protocol for grass pea (Lathyrus sativus L.) was optimized using different concentrations and combinations of growth regulators. Direct shoot regeneration obtained through shoot organogenesis from different explants of grass pea cultured on MS medium supplemented with Gamborg B5 vitamin containing 6-benzylaminopurine (BAP), Thidiazuron (TDZ) and ?-naphthalene acetic acid (NAA). Highest percentage of shoots were obtained at 4.0 mg/l of BAP on nodal explants. Stunted multiple shoots were developed from nodal explants while 1.5 mg/l TDZ was used. About 56% of direct shoots were also obtained, while the combination of BAP (4.0 mg/l) and NAA (0.5 mg/l) were used. Regenerated plantlets were rooted most effectively (40%) in rooting medium containing half strength of MS basal medium containing 1.0 mg/l NAA. Well rooted plantlets were further successfully acclimatized to ambient humidity level and grown in controlled environment until hardening.Jahangirnagar University J. Biol. Sci. 4(2): 1-8, 2015 (December)
Highlights
Grass pea (Lathyrus sativus L.) is a leguminous crop plant cultivated extensively in various parts of the world especially the food-deficit countries due to its qualities of being a very cheap source of diet protein (Kenicer et al, 2005)
Different concentrations (0.5, 1.0 and 2.0 mg/l) of auxin type hormones e.g. indole butyric acid (IBA) and naphthalene acetic acid (NAA) added to half-strength Murashige and Skoog (MS) medium separately for root initiation and elongation of both shoots and roots
Node and internodal segments were cultured on MS media with B5 vitamin (MS-B5) medium supplemented with benzylaminopurine (BAP) or thidiazuron (TDZ) alone and in combinations with BAP and auxins, α–naphthalene acetic acid (NAA) in varying concentrations and combinations
Summary
Grass pea (Lathyrus sativus L.) is a leguminous crop plant cultivated extensively in various parts of the world especially the food-deficit countries due to its qualities of being a very cheap source of diet protein (Kenicer et al, 2005). For this study explants e.g. leaflets, cotyledonary nodes, and internodal segments were used as explant’s source from in vitro grown grass pea seedlings for direct shoot organogenesis. In vitro Regeneration, Grass Pea hormone e.g. α-Naphthalene Acetic Acid (NAA) (0.0+0.0, 4.0+0.5, 4.0+1.0, 4.0+2.0) added to the MS medium for shoot primordia initiation and its elongation.
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