In vitro Recovery of Dengue Viruses from Naturally Infected Human Beings and Arthropods

Abstract

IN the period which has followed the discovery of the mouse neurovirulence of dengue viruses types 1 and 21–3, intracerebral inoculation of the suckling mouse has remained the sole, though inefficient, laboratory method for the recovery of viruses of the dengue group4,5. Despite successful attempts to grow mouse adapted dengue viruses in several cell cultures, primary isolation and identification of dengue viruses entirely by an in vitro method have not been reported. This communication describes the recovery and identification of dengue viruses of several antigenic types in a stable line of grivet monkey kidney cells, Cercopithecus aethiops.