In-vitro radical scavenging activity of Daucus carota L. extracts

Abstract

Background and objective Antioxidants play a vital role against the harmful effect caused by oxidative stress. The aim of the current study was to assess the antioxidant potential of methanol extracts of Daucus carota L. callus addend with an amino acid precursor, l-phenylalanine, under light and dark conditions. Materials and methods Callus cultures of petiole, stem, and root explants of D. carota L. in-vitro seedlings were implanted on calli maintenance medium fortified with 500 and 1000 mg/l l-phenylalanine as an amino acid precursor and then were incubated under light and dark conditions. The various prepared concentrations of D. carota L. callus crude extracts of petiole, stem, and root explants by maceration with 85% methanol were screened for possible antioxidant activity using 2, 2′-diphenyl 1-picrylhydrazyl radical scavenging test. Results and conclusion The present study re-cultured the induced calli from petiole, stem, and root explants of D. carota L. on the best selected medium [MS-medium supplemented with 1 mg/l benzylaminopurine (BAP)+2 mg/l naphthaleneaceticacid (NAA)] for callus cultures added with l-phenylalanine. The different concentrations (2, 4, 6, 8, 10, 12, 14, 16, and 18 mg/ml) of the prepared callus cultures extracts of stem, root, and petiole explants of D. carota L. have been tested for antioxidant effects. The results revealed that, under dark condition, 12 mg of petiole extract concentration exhibited the least concentration of methanol crude extract recording the greatest antioxidant activity (124.71%) on medium containing 1000 mg/l phenylalanine in comparison with other callus extracts. The results of this investigation revealed that calli of D. carota L. petiole explant should be incubated under dark condition, and their methanol crude extract ought to be used at a concentration of 12 mg. Hence, D. carota L. extract should be considered as a new source of natural antioxidant.