In-vitro quantitative measurement and analysis of the photosensitivity of cells to a weak pulse laser.

Abstract

Light can trigger electrical activity in certain types of cells, and is considered to be a better means of biological regulation than electrical stimulation in the future. Due to the specificity and selectivity of natural cells' photoresponse to optical signals, constructing an applicable method to explore which kinds of cells have photosensitivity and which bands of light could induce its photoresponse most effectively, is of great significance for lights' medical applications. This paper firstly proposed a universal and operable system and corresponding method to quantitatively measure and analyze photosensitivity of cells in vitro to weak pulse laser, which is constructed with Ca2+ imaging module, adjustable laser lights module and laser positioning module. With the measurement system and method, the photosensitive effects of the natural spiral ganglion cells (SGCs) of mice are tested systemantically. Then a new photoresponse band of light (453 nm, 300 µs) is found for SGCs, and its minimum threshold is measured as 5.3 mJ/cm2. The results verify that the proposed method is applicable to screen the cells with photosensitive response, as well as to measure and analyze the working optical parameters, thus is beneficial for the optical biophysics and photobiology.