Abstract
Abstract The antioxidant sodium diethyldithiocarbamate (DDC) was evaluated for use as a protectant of indole-3-acetic acid (IAA) during application onto cellulose thin layer strips as well as during subsequent ascending chromatographic development. An IAA standard of 5 μg, applied directly over a 100 μg pretreatment spot of DDC at the origin, revealed eightfold more auxin activity than did 5 μg of IAA alone as determined by the Avena coleoptile bioassay. However, DDC alone at this dosage produced an inhibitory biological response at the same chromatographic zone where auxin banded in an isopropanol, ammonia, water (10:1:1 by volume) solvent system. Reduction of the DDC dosage to 1 μg spot−1 gave threefold greater net auxin activity than did 100 μg DDC, and 20% better protection than did 0.5 μg DDC, while eliminating its own inhibitory action at this lower dosage level.
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