Abstract
In this elaboration, effective methods of in vitro propagation of a Paulownia tomentosa × Paulownia fortunei hybrid are presented, and the variable costs of commercial production evaluated. Plant regeneration of the P. tomentosa × P. fortunei hybrid was achieved through organogenesis in nodal explants. Different concentrations of BAP (6-benzylaminopurine), 0.2, 0.5, 1 mg/L, and light conditions were investigated. The best results were obtained using a half-strength MS medium containing 0.5 mg/L BAP. In standard light conditions, 2 shoots were grown with 3.5 culturable nodes on each, and in 70% reduced light, 2 new shoots were grown with 6 culturable nodes on each. Rooting was successfully achieved when using a hormone-free half-strength MS medium containing vitamin, and 2% sucrose with 95% efficiency. Acclimatization and survival were shown to be 90% in regenerated plants. The cost of production of a single plant of P. tomentosa × P.fortunei hybrid grown in standard light conditions was $0.084 and $0.082 when grown in 70% reduced light where only variable costs were considered. Two major factors affecting P. tomentosa × P fortunei hybrid micropropagation is labor, materials and chemicals. Focusing on reducing this cost can highly lower plantlet price.
Highlights
A new, very perspective plant, Paulownia has been introduced into Polish nursery production the last few years
The explants used for in vitro culture initiation were meristems excised from nodal segments and shoot tips of one year old plants that were clones of a P. tomentosa × P. fortunei hybrid
Explants were placed in half-strength MS medium with vitamins [10] containing 1mg/L 6-benzylaminopurine (BAP), 2% sucrose, 0.2 mL/L Plant Preservative Mixture (PPM, Plant Cell Technology Inc, Washington, DC 20036, USA) and 7 g/L plant agar according to Venkateswarlu et al [11]
Summary
A new, very perspective plant, Paulownia has been introduced into Polish nursery production the last few years. This genus includes nine species and numerous interspecific hybrids. P. elongata is a fast-growing species with a straight trunk, medium crown and suitable for warm climates. From an economic point of view, is that Paulownia does not need re-planting because it grows from stumps after being harvested, and the process can be repeated several times. These plants were used for afforestation [3], for the
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