In vitro propagation of Vitis vinifera L. cv. ‘Monastrell’

Abstract

Normal 0 false false false ES X-NONE X-NONE Background: A protocol for micropropagation of the grape ( Vitis vinifera L.) cultivar ‘Monastrell' was developed. Initial plant material was based on sanitary selection of grapevine plants performed by real-time RT-PCR, to confirm the absence of Grapevine fanleaf virus (GFLV), Arabis mosaic virus (ArMV), Grapevine leafroll-associated virus 1 (GLRaV-1), Grapevine leafroll-associated virus 3 (GLRaV-3), and Grapevine fleck virus (GFkV). Results: The effects of the salt composition (comparing Lloyd and McCown woody plant medium and Murashige and Skoog medium ½ macronutrients) and the growth regulator benzylaminopurine (BAP), at 0 and 8.9 µM, on the multiplication step were evaluated using nodes as explants. The most-efficient procedure consisted of bud induction in the medium with Lloyd and McCown woody plant salts and 8.9 µM BAP for 30 days along with elongation in cytokinin-free medium for 60 days, which gave 22 nodes/explant (174 plants/initial plant). A second cycle of multiplication in a medium without the growth regulator for another 60 days could give around 10,000 nodes; this quantity of rooted plants could be obtained after another two months of culture. All plants acclimatized after the second cycle of multiplication were successfully transferred to soil. Conclusion: We developed an optimal protocol for V. vinifera cv. Monastrell micropropagation, the first described for this cultivar. /* Style Definitions */ table.MsoNormalTable {mso-style-name:Tabla normal; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:Calibri,sans-serif; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:Times New Roman; mso-bidi-theme-font:minor-bidi; mso-ansi-language:ES; mso-fareast-language:ES;}