Abstract
In this work, the effect of various hormones on in vitro multiplication and elongation of Paeonia suffruticosa is studied and several morphological and physiological abnormalities induced by them are described. Among the five cytokinins tested, only benzylaminopurine (BAP) allowed the development of axillary buds. However, the concentration of this hormone and the age of the shoots used must be taken into account to avoid vitrification. Gibberellic acid (GA 3) alone in the culture medium allowed neither shoot elongation nor leaf and axillary bud formation or development. The combination of BAP with GA 3 increased the multiplication rate as compared with BAP alone and allowed shoot elongation. This hormonal combination sometimes caused apical and leaf necroses, especially under low Ca 2+ concentrations. Consequently, a BAP-GA 3 medium is not recommended for regular use, although it can be interposed in a culture series on the BAP medium, to stimulate periodic axillary bud development.
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